Biochemical and instrumental aspects of fibre-optic biosensor development are considered in this study. A sensing process must be capable of transducing the selective chemical binding event into an optical signal. A system showing sensitivity, high selectivity and reversibility was derived from the fluorescence detection of acetylcholine receptor binding to agonist. Fluorescence enhancement was seen for systems where a fluorophore was non-specifically labelled to the receptor/membrane structure and also where only the lipid was fluorescent.
The use of intensity ratios at different wavelengths allowed for internal calibration for instrumental drift. Instrumentation for optrode development in an intrinsic configuration was demonstrated by fluorescence from Pyrenelabelled concanavalin A adsorbed to the surface of an optical fIbre.
Brown, R.S., Krull, U.J. and Hougham, B.D., 1988. Selective Biochemical Interactions of Acetylcholine Receptor on the Surface of an Optical Fibre. In Biotechnology Research and Applications (pp. 195-204). Springer Netherlands.
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Category: Material & Chemical