To image microstructures within tissue in detail using conventional light microscopy it is necessary to process the tissue and obtain thin (/spl ap/5 /spl mu/m) physical sections by using destructive physical sectioning techniques. This article describes a microscopy technique, using near-infra-red light (700-1500 nm) as the source, which is able to image thin sections of thick-tissue specimens nondestructively. Images of contiguous two-dimensional thin sections obtained serially can be combined to generate a three-dimensional image of the structures being imaged.
Murthy, M.S.N., Jones, M.G., Kulka, J., Davies, J.D., Halliwell, M., Jackson, P.C., Bull, D.R. and Wells, P.N.T., 1995. Scanning confocal near-infra-red microscopy: a new microscopy technique for three-dimensional histopathology. Engineering Science and Education Journal, 4(5), pp.223-230.
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Categories: Photonics & Optoelectronics